DNA barcoding of simulium asakoae (Diptera: Simuliidae) from Northern Thailand
Document Type
Article
Publication Date
9-1-2020
Abstract
Previous studies suggested the presence of species complex in the so-called Simulium asakoaeTakaoka & Davies (Diptera: Simuliidae) in Thailand due to its high morphological variability and genetic divergence. To investigate whether the true S. asakoae is present inThailand, we performed a detailed morphological identification of S. asakoae and compared its DNA barcodes with the morphospecies S. asakoae from Myanmar and the typical S. asakoae from Malaysia. Phylogenetic analysis revealed theThai materials analyzed in this study were indeed genetically similar with those from Myanmar and Malaysia, though genetic distances 0-2.27% were observed. We tentatively regard this divergence as intraspecific variation, and the automatic barcode gap discovery analysis further supports them as a single species.
Keywords
DNA barcode, COI gene, Black fly, Vector, Thailand
Divisions
tidrec
Publication Title
Journal of Medical Entomology
Volume
57
Issue
5
Publisher
Oxford Univ Press Inc.
Publisher Location
JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA