Chemopreventive activity of Tualang honey against oral squamous cell carcinoma-in vivo
Document Type
Article
Publication Date
5-1-2020
Abstract
Objective. The aim of this study was to evaluate the chemopreventive activity of Malaysian jungle Tualang honey (TH) after oral carcinogenesis induced with 4-nitroquinoline 1-oxide (4 NQO). Study Design. A total of 28 male Sprague-Dawley (SD) rats were distributed into 4 groups as follows: group 1 (nontreated group); group 2 (control), which received 4 NQO during 8 weeks in drinking water only; and groups 3 and 4, which received 4 NQO for 8 weeks in drinking water and treated with TH 1000 mg/kg and 2000 mg/kg by oral gavage for 10 weeks. All rats from all experiments were sacrificed after 22 weeks, and the incidence of oral neoplasms and histopathologic changes were microscopically evaluated. Moreover, immunohistochemical expression was analyzed in tongue specimens by using image analysis software. The expression of particular genes associated with oral cancer were assessed by using RT2 Profiler PCR Array (Qiagen, Germantown, MD). Results. TH significantly reduced the incidence of oral squamous cell carcinoma (OSCC) and suppressed cancer cell proliferation via diminishing the expression of CCND1, EGFR, and COX-2. Furthermore, TH preserved cellular adhesion (epithelial polarity) through overexpression of beta-catenin and e-cadherin and inhibited the OSCC aggressiveness by downregulating TWIST1 and RAC1. Conclusions. Our data suggest that TH exerts chemopreventive activity in an animal model in which oral cancer was induced by using 4 NQO.
Keywords
Animals, Carcinoma, Squamous Cell, Honey, Male, Mouth Neoplasms, Rats, Rats, Sprague-Dawley, Tongue Neoplasms
Divisions
Dentistry,fac_med
Funders
Ministry of Higher Education, Malaysia [Grant No: PG 253-2015 B],Universiti Malaya [Grant No: Fp 030-2015 A]
Publication Title
Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics
Volume
129
Issue
5
Publisher
Mosby Inc.
Publisher Location
STE 800, 230 PARK AVE, NEW YORK, NY 10169 USA