Amelioration of hyperglycemia-induced oxidative damage in ARPE-19 cells by myricetin derivatives isolated from Syzygium malaccense
Document Type
Article
Publication Date
4-1-2020
Abstract
Myricetin derivatives (F2) isolated from leaf extract of Syzygium malaccense (Malay apple) which contains myricitrin predominantly, could potentially serve as functional food ingredient based on previous findings. The present study aimed to investigate the protective effects of F2 against hyperglycemia-induced oxidative stress in ARPE-19 (retinal pigment epithelium) cells; a diabetic retinopathy (DR) model. F2 showed effective inhibition of advanced glycation end products (AGE) formation. The derivatives attenuated high glucose (30 mM)-induced stress condition in ARPE-19 cells by reducing intracellular reactive oxygen species (ROS). At transcriptional level, F2 activated Nrf2 pathway besides upregulating antioxidant enzymes and other protective factors. In addition, protective effect of F2 against formation of AGE correlated with its ability to downregulate gene expression of inflammatory factor, NFkB1 and receptor for AGE. The findings showed that F2 was effective in preventing oxidative damage-induced by high glucose in ARPE-19 and could be developed as an adjuvant to manage DR.
Keywords
Antiglycation, Diabetic retinopathy, Hyperglycemia, Myricitrin, Oxidative stress, Syzygium malaccense
Divisions
fac_med,biomedsc
Publication Title
Journal of Functional Foods
Volume
67
Publisher
Elsevier
Publisher Location
RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS