A LEGO inspired fiber probe analytical platform for early diagnosis of Dengue fever
Document Type
Article
Publication Date
4-1-2020
Abstract
Based on the concept of LEGO toys, a fiber probe analytical platform (FPAP) was developed as a powerful diagnostic tool offering higher sensitivity in detection of infectious agents compared to established methods. Using the form and the function of LEGO toys, this protocol describes a fiber-based, 96-well plate, which suspends a new class of chemically-designed, electrospun fibers within the assay. This clamping strategy allows both sides of the developed fiber mats to interact with biomolecules within the assay thus benefiting from the tailored chemical and physical properties of these fiber-based bioreceptors in attracting the biomolecules to the surface. The fabrication method of FPAP involves one-step electrospinning of the chemically designed fibers, 3D printing of the LEGO-like probing segments, and assembly of the device followed by ELISA procedure. FPAP follows the same principles of operation as that of a conventional enzyme linked immunosorbent assay (ELISA), therefore, it can be run by lab technicians, expert in ELISA. FPAP was used for early diagnosis of Dengue fever and provided an 8-fold higher sensitivity while the limit of detection (LOD) was recorded to be in femto-gram per milliliter range which is significantly low when compared to other existing techniques or conventional assay. This platform allows different types of paper/fiber bio-receptive platforms to be incorporated within the design that promises simultaneous recognition of multiple infectious agents.
Keywords
Paper-/fiber-based analytical platforms, Dengue detection, Surface functional groups, Integrated bio-sensing platform, Electrospinning
Divisions
fac_eng,biomedengine
Funders
Nano Sensors and Devices Focus Group, School of Engineering and Sciences,Tecnologico de Monterrey [Grant No: 002EICII01],Ministry of Higher Education, Malaysia,Frontiers Foundation [Grant No: FG021-17AFR],Instituto Tecnológico y de Estudios Superiores de Monterrey
Publication Title
Materials Science and Engineering C-Materials for Biological Applications
Volume
109
Publisher
Elsevier
Publisher Location
RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS