Effects of gamma radiation treatment on three different medicinal plants: Microbial limit test, total phenolic content, in vitro cytotoxicity effect and antioxidant assay
Document Type
Article
Publication Date
3-1-2020
Abstract
The aim of this study is to evaluate the effects of gamma radiation treatment on three medicinal plants, namely Euodia malayana, Gnetwn gnemon and Khaya senegalensis at two different forms; methanol leaf extracts and dried leaves respectively. Experimental procedure: The microbial limit test (MLT) studies indicated the suitable dosage of minimum and maximum gamma irradiation for leaf extracts as well as dried leaves of all the tested medicinal plants. Quantitative analysis of total phenolic content (TPC) analysis is based on calorimetric measurements determined using the Folin-Ciocalteu reagent with gallic acid (GA) used as the reference. In vitro cytotoxicity assay by using fibroblast (L929) cell lines was performed on each plant to determine the toxicity effect which sodium dodecyl sulfate (SDS) as the positive control. DPPH (2,2-diphenyl-1-picryl-hydrazyl) assay was conducted by using vitamin C and GA as the positive controls to determine the antioxidant property of each plant. Results and conclusion: The MLT analysis indicated that the suitable dosage gamma irradiation for leaf extracts was 6-12 kGy and dried leaves were 9-13 kGy. The amount of GA concentration in each plant increased significantly from 30-51 mg GAE g(-1) before treatment to 57-103 mg GAE g(-1) after treatment with gamma radiation. This showed no significant effect of in vitro cytotoxicity activity before and after treatment with gamma irradiation in this study. Effective concentration (EC50) values of Khaya senegalensis plant reduced significantly (P <= 0.005) from 44.510 mu g/ml before treatment to 24.691 mu g/ml after treatment with gamma radiation, which indicate an increase of free radical scavenging activity.
Keywords
Medicinal plants, Microbial limit test, Total phenolic content, In vitro cytotoxicity, DPPH (2 2-Diphenyl-1-picryl-hydrazyl)
Divisions
Science
Funders
Ministry of Energy, Science, Technology, Environmental and Climate Change (MESTECC),undamental Research Grant Scheme [FP0813B023]
Publication Title
Applied Radiation and Isotopes
Volume
157
Publisher
Elsevier
Publisher Location
THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
Additional Information
16th Latin American Conference of Analysis by X-Ray Techniques (SARX) / 6th Conference of Medical Physics in the Frontier (JFMF), Pucon, PEOPLES R CHINA, NOV 04-07, 2018