Complete genome sequence of a novel polyhydroxyalkanoate (PHA) producer, Jeongeupia sp. USM3 (JCM 19920) and characterization of its PHA synthases
Document Type
Article
Publication Date
3-1-2020
Abstract
A novel polyhydroxyalkanoate (PHA)-producing bacterium, Jeongeupia sp. USM3 (JCM 19920) was isolated from the limestone soil at Gua Tempurung, Perak, Malaysia. This is the first report on the complete genome sequence for the genus Jeongeupia. This genome consists of a circular chromosome with a size of 3,788,814 bp and contains 3557 genes. Two PHA synthase (phaC) genes encoding for the key enzyme in the polymerization of PHA monomers and other PHA-associated genes were identified from the genome. Phylogenetic analysis of the PhaC protein sequences has revealed that both PhaC1 and PhaC2 of Jeongeupia sp. USM3 are categorized as Class I PHA synthases with 56% similarity to each other. Both of the PHA synthase genes of this isolate were cloned and heterologously expressed in a PHA mutant strain Cupriavidus necator PHB(-)4. The ability of the transformants to accumulate PHA showed that both PhaC1 and PhaC2 were functional.
Keywords
Alcaligenes-eutrophus H16, Beta-hydroxybutyric acid
Funders
USM-RIKEN International Centre for Aging Science [1001/PBIOLOGI/870033]
Publication Title
Current Microbiology
Volume
77
Issue
3
Publisher
Springer Verlag (Germany)
Publisher Location
ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES