Spectroscopic (UV/VIS, Raman) and Electrophoresis Study of Cytosine-Guanine Oligonucleotide DNA Influenced by Magnetic Field
Document Type
Article
Publication Date
1-1-2016
Abstract
Studying the effect of a magnetic field on oligonucleotide DNA can provide a novel DNA manipulation technique for potential application in bioengineering and medicine. In this work, the optical and electrochemical response of a 100 bases oligonucleotides DNA, cytosine- guanine (CG100), is investigated via exposure to different magnetic fields (250, 500, 750, and 1000 mT). As a result of the optical response of CG100 to the magnetic field, the ultra-violet-visible spectrum indicated a slight variation in the band gap of CG100 of about 0.3 eV. Raman spectroscopy showed a significant deviation in hydrogen and phosphate bonds' vibration after exposure to the magnetic field. Oligonucleotide DNA mobility was investigated in the external electric field using the gel electrophoresis technique, which revealed a small decrease in the migration of CG100 after exposure to the magnetic field.
Keywords
DNA, Electrophoresis, Magnetics, Spectrophotometry, Ultraviolet, Spectrum Analysis, Raman
Divisions
PHYSICS
Funders
PPP-BKP grant BK003-2015,UM/MOHE High Impact Research Grant allocation of F000006-21001,HIR grants (J-21002-73823 and UM.C/HIR/MOHE/SC/06),FRGS grant (FP004/2013A)
Publication Title
PLoS ONE
Volume
11
Issue
3
Publisher
Public Library of Science