Differential gene expression analysis of Secreted in Xylem (SIX) genes from Fusarium oxysporum f. sp. cubense tropical race 4 in Musa acuminata cv. Berangan and potential application for early detection of infection
Document Type
Article
Publication Date
1-1-2020
Abstract
Homologues of Secreted in Xylem (SIX) genes, a group of known fungal effectors, were here identified in the genome of Fusarium oxysporum f. sp. cubense tropical race 4 (FocTR4). Expression of FocSIX genes was analyzed in FocTR4 vegetative mycelia and FocTR4-infected banana roots using quantitative reverse transcription PCR (qRT-PCR). Differential gene expression of all FocSIX genes identified in the genome was assessed over time in banana roots inoculated with FocTR4. Two groups of FocSIX genes expression pattern were found; Group I (FocSIX8a, FocSIX1b, FocSIX1c and FocSIX2) represents FocSIX genes that were only expressed in planta, and Group II consists of the remaining FocSIX genes (FocSIX1a, FocSIX6, FocSIX9 and FocSIX13) that were expressed in vitro and in planta. With the FocTR4 genomic information, Group I members can potentially serve as biomarkers for the early detection of Fusarium wilt infection in banana. © 2019, The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature.
Keywords
Banana, Fusarium oxysporum f. sp. cubense, Fusarium wilt, Gene expression, Secreted in Xylem (SIX) gene
Divisions
InstituteofBiologicalSciences,cebar
Funders
University of Malaya HIR-MOE (Cycle 3) Grant-UM.C/625/1/HIR/MOE/SC/14/4,Postgraduate Research Grant (PPP)-PG178-2016A,BKP078-2016,RU006-2017
Publication Title
Journal of General Plant Pathology
Volume
86
Issue
1
Publisher
Springer