The emergence of colistin-resistant Klebsiella pneumoniae strains from swine in Malaysia
Document Type
Article
Publication Date
1-1-2019
Abstract
Objective: Colistin is the last line of therapy for infections caused by multidrug-resistant Gram-negative bacteria. The objective of this study was to determine the phenotypic and genotypic characteristics of colistin-resistant Klebsiella pneumoniae (K. pneumoniae) isolated from swine samples in Malaysia. Methods: A total of 46 swine K. pneumoniae strains isolated from 2013–2015 in Malaysia were analysed for the production of extended-spectrum β-lactamases and carbapenemase. The resistance traits and genetic diversity of these strains were characterised by polymerase chain reaction, conjugation, plasmid analysis, and pulsed-field gel electrophoresis. Results: Nineteen of 46 strains were multidrug resistant while 13 were resistant to colistin. The majority of colistin-resistant strains harboured blaTEM gene (92.3%), followed by blaSHV (69.23%), blaCTXM-1 (38.46%), and blaMCR-1 (23.08%). All three colistin-resistant strains had transferable plasmids and the colistin resistance gene blaMCR-1. Genotyping by pulsed-field gel electrophoresis showed high genetic diversity among the K. pneumoniae and that the colistin-resistant K. pneumoniae strains were heterogenous. Conclusion: It is believed that this is the first report of colistin-resistant K. pneumoniae among swine strains associated with mcr-1 plasmid in Malaysia. Due to the emergence of β-lactam, carbapenem and colistin resistance, the use of colistin in animal husbandry and agriculture should be avoided to prevent treatment failure. © 2019 International Society for Chemotherapy of Infection and Cancer
Keywords
Colistin, Genetic diversity, Klebsiella pneumoniae, Multidrug resistance, Swine
Divisions
fac_med,InstituteofBiologicalSciences
Funders
University of Malaya Postgraduate Research Grant — ( PG072-2014B ),High Impact Research (HIR) Grant ( UM.C/625/1/HIR/MOHE/CHAN/11/02 )
Publication Title
Journal of Global Antimicrobial Resistance
Volume
17
Publisher
Elsevier