The authentication of virgin coconut oil from grape seed oil and soybean oil using ftir spectroscopy and chemometrics
Document Type
Article
Publication Date
1-1-2019
Abstract
Objective: The objective of this study was to develop Fourier transform infrared (FTIR) spectroscopy in combination with chemometrics of multivariate calibration and discriminant analysis (DA) for the authentication of virgin coconut oil (VCO) from grape seed oil (GSO) and soybean oil (SO). Methods: FTIR spectra of VCO, GSO, SO and its binary mixture of VCO-SO, and VCO-GSO were scanned at mid-infrared region (4000-650 cm-1 Results: The results showed that partial least square (PLS) calibration using absorbance values at combined wavenumbers of 1200-900 and 3027-2985 cm) using attenuated total reflectance technique. The wavenumbers were selected based on its capability to provide the best prediction models for quantification and classification of adulterants in VCO assisted by multivariate calibrations and DA, respectively. -1 revealed reliable method for quantification of GSO in VCO, as indicated by high value of coefficient of determination (R2) and low value of root mean square of calibration (RMSEC) and root mean square error of prediction (RMSEP). PLS using FTIR spectra at the combined wavenumbers of 1200-1000 and 3025-2995 cm-1 Conclusion: FTIR spectroscopy in combination with chemometrics of multivariate calibration and DA offered effective tools for the authentication of VCO. was suitable for quantitative analysis of SO in VCO. DAwas also successfully used for classification of VCO and VCO added with adulterants of GSO and SO. © 2019 The Authors. Published by Innovare Academic Sciences Pvt Ltd.
Keywords
Virgin coconut oil, Chemometrics, Authentication, FTIR spectroscopy
Divisions
nanotechnology
Funders
Ministry of Research and Higher Education for the financial support to the first author via program World Class University 2018 in Universiti Malaya
Publication Title
International Journal of Applied Pharmaceutics
Volume
11
Issue
2
Publisher
Innovare Academic Sciences Pvt. Ltd. India