Micropropagation and Cellular Behaviour Changes During in vitro Flowering of Impatiens balsamina
Document Type
Article
Publication Date
1-1-2019
Abstract
In this study, the micropropagation of Impatiens balsamina was established from stem and shoot explants. The effects of GA3 and glutathione on the morphogenesis of this species were also investigated, in order to induce in vitro flowering. It was found that the optimum in vitro plant regeneration was achieved on MS medium supplemented with 1.0 mg L-1 GA3 and in vitro flowering was also obtained from the same medium after 4 weeks of culture. To understand cellular behavior during in vitro flowering, Mitotic Index (MI), chromosome counts, measurement of mean cell and nuclear areas, DNA measurements and ploidy levels were analyzed from in vivo plants, in vitro grown plants and plantlets that flowered in vitro. The chromosome count was the same for all, 2x=2n=14 or n=7. However, it was observed that in vitro flowering plants of Impatiens balsamina had the highest percentage of polyploid cells (30.7%), based on a histogram plotted by the AxioVision 4.7 software. It was found that plant growth regulators, especially GA3, increased the polyploidy level of the meristematic root cells. © 2019, Sociedade Brasileira da Ciencia das Plantas Daninha. All rights reserved.
Keywords
Cellular behavior and acclimatization, Flowering response, Gibberellic acid, Glutathione, Regeneration
Divisions
InstituteofBiologicalSciences
Funders
Institute of Research Management and Monitoring (IPPP) of the University of Malaya (PG044/2013A)
Publication Title
Planta Daninha
Volume
37
Publisher
Sociedade Brasileira da Ciencia das Plantas Daninha