Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane
Document Type
Article
Publication Date
1-1-2019
Abstract
In this work, a chitosan-modified nanofiber membrane was fabricated and used to examine the permeation characteristics of C-phycocyanin (CPC) obtained from Spirulina platensis. The effects of NaCl concentration (0.1–1.0 M), chitosan coupling pH (6–8), chitosan coupling concentration (0.1–3.0%), algal solution pH (6–8), algal mass concentration (0.1–1.0% dw/v), and membrane flux (4.08 × 10 −2 –2.04 × 10 −1 mL/min·cm 2 ) on the penetration performance of the membrane for CPC were investigated. The results show that the order of binding selectivity of the membrane for these proteins is contaminating proteins (TP) > allophycocyanin (APC) > CPC. TP and APC molecules were more easily adsorbed by the chitosan-modified membrane, and the CPC molecules most easily penetrated the membrane without being adsorbed, enhancing CPC purity. The purification factor and total mass flux were 3.3 fold and 66%, respectively, in a single step. © 2019 Elsevier B.V.
Keywords
Chitosan-modified nanofiber membrane, Purity, C-phycocyanin, Spirulina platensis, Negative chromatography
Divisions
PHYSICS,InstituteofBiologicalSciences
Funders
Ministry of Science and Technology, Taiwan (grant numbers MOST 105-2622-E-131-004-CC3, 104-2221-E-131-021, and 103-2221-E-131-023)
Publication Title
International Journal of Biological Macromolecules
Volume
132
Publisher
Elsevier
Additional Information
Doctoral Degree (phd). Malcolm Tang Siong Yii.