Ultrasmall superparamagnetic Fe3O4 nanoparticles: honey-based green and facile synthesis and in vitro viability assay
Document Type
Article
Publication Date
1-1-2018
Abstract
Introduction: In the present research, we report a quick and green synthesis of magnetite nanoparticles (Fe 3 O 4 -NPs) in aqueous solution using ferric and ferrous chloride, with different percentages of natural honey (0.5%, 1.0%, 3.0% and 5.0% w/v) as the precursors, stabilizer, reducing and capping agent, respectively. The effect of the stabilizer on the magnetic properties and size of Fe 3 O 4 -NPs was also studied. Methods: The nanoparticles were characterized by X-ray diffraction (XRD) analysis, field emission scanning electron microscopy, energy dispersive X-ray fluorescence, transmission electron microscopy (TEM), vibrating sample magnetometry (VSM) and Fourier transform infrared spectroscopy. Results: The XRD analysis indicated the presence of pure Fe 3 O 4 -NPs while the TEM images indicated that the Fe 3 O 4 -NPs are spherical with a diameter range between 3.21 and 2.22 nm. The VSM study demonstrated that the magnetic properties were enhanced with the decrease in the percentage of honey. In vitro viability evaluation of Fe 3 O 4 -NPs performed by using the MTT assay on the WEHI164 cells demonstrated no significant toxicity in higher concentration up to 140.0 ppm, which allows them to be used in some biological applications such as drug delivery. Conclusion: The presented synthesis method can be used for the controlled synthesis of Fe 3 O 4 -NPs, which could be found to be important in applications in biotechnology, biosensor and biomedicine, magnetic resonance imaging and catalysis.
Keywords
honey, Fe3O4 nanoparticles, green synthesis, transmission electron microscopy, magnetic properties, in vitro, viability, MTT assay, WEHI164 cells
Divisions
CHEMISTRY,nanotechnology
Funders
University of Malaya under the Equitable Society Research Cluster (ESRC) research grant GC001C-14SBS
Publication Title
International Journal of Nanomedicine
Volume
13
Publisher
Dove Medical Press