Transient Expression of an Immunogenic Envelope Attachment Glycoprotein of Nipah Virus in Nicotiana benthamiana
Document Type
Article
Publication Date
1-1-2018
Abstract
The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems are gaining recognition as a viable alternative for the production of vaccine antigens. In this study, we expressed the Nipah virus G protein heterologously in Nicotiana benthamiana using an agroinfiltration approach. The highest expression of recombinant G protein in N. benthamiana at RNA and protein levels was detected on day 9 post-infiltration. Western blot analysis demonstrated that the purified G protein reacted specifically with rabbit anti-Nipah Virus serum, indicating its potential for vaccine use.
Keywords
Biotechnology, KDEL, plants, tobacco PR1a, vaccine
Divisions
InstituteofBiologicalSciences
Funders
High Impact Research Chancellery Grant UM.C/625/1/ HIR/MOHE/SCI/14,CEBAR grant RU006-2017 from the University of Malaya,ABI fund under project code RB1086AB10 from the Ministry of Science, Technology and Innovation, Malaysia
Publication Title
Sains Malaysiana
Volume
47
Issue
3
Publisher
Penerbit Universiti Kebangsaan Malaysia