Successful cleavage of cloned goat embryos using ear fibroblast cell and fetal fibroblast cell as donor karyoplast in interspecies SCNT
Document Type
Article
Publication Date
1-1-2018
Abstract
The efficiencies in producing cloned ruminant embryos and subsequent production of offspring are still low. The study was conducted to produce cloned goat and cattle embryos using inter- and intraspecies SCNT techniques. This study involved 3 different types of donor karyoplast using goat and cattle oocyte as a recipient cytoplast to produce cloned goat and cattle embryos. The results showed that fetal fibroblast cell (FFC) in interspecies SCNT gave significantly higher 2-cell (64.40 vs. 38.43%), 4-cell (54.24 vs. 24.60%), 8-cell (36.82 vs. 14.54%) and morula (22.10 vs. 7.90%) cloned goat embryos than ear fibroblast cell (EFC). As for intraspecies SCNT using cumulus cell (CC) as a donor karyoplast to produce cloned cattle and goat embryos, the values for cleavage rates were not significantly different which were; 53.57 vs. 57.17%, 33.17 vs. 46.40%, 22.15 vs. 27.30% and 11.90 vs. 15.59%, respectively for all embryo stages. Our results showed that cloned goat and cattle embryos could be produced using different types of donor karyoplast in intra- and interspecies SCNT. However, for goat-cattle interspecies SCNT, FFC was more efficient to produce cloned goat embryos compared to EFC.
Keywords
Cattle, Donor karyoplast, Goat, Intraspecies SCNT, Interspecies SCNT, Recipient cytoplast
Divisions
InstituteofBiologicalSciences
Funders
e-Science grant (MOSTI) SF015-13
Publication Title
Indian Journal of Animal Sciences
Volume
88
Issue
9
Publisher
Indian Council of Agricultural Research