Panel of serum protein biomarkers to grade the severity of traumatic brain injury
Document Type
Article
Publication Date
1-1-2018
Abstract
The Glasgow Coma Scale (GCS), which classifies patients into mild, moderate or severe traumatic brain injury (TBI), is a system used to prioritize treatment and prognosticate the severity of head injury. In this study, sera of patients with various stages of TBI, as well as control subjects, were analyzed to screen for proteins that may be used to complement the GCS system. By subjecting pooled serum samples to iTRAQ analysis for quantitative comparison of protein abundance, and attesting their altered levels using ELISA, we have detected increased levels of serum amyloid A, C-reactive protein, leucine-rich alpha-2-glycoprotein, lipopolysaccharide-binding protein, fibronectin, vitronectin and alpha-1-antichymotrypsin in patients across all strata of TBI relative to the controls. However, kininogen was decreased only in moderate and severe TBI, whereas apolipoprotein E and zinc-alpha-2-glycoprotein were only increased in severe TBI. Hence, we propose a panel of serum biomarkers, which if analyzed within 24 h of the injury, can be used to diagnose patients with TBI into mild, moderate or severe stratification objectively, thus complementing the traditional GCS.
Keywords
Biomarker, Glasgow Coma Scale, iTRAQ, Serum proteins, Traumatic brain injury
Divisions
fac_med
Funders
Research grants RG249/10HTM and PV064/2011A from the University of Malaya, Kuala Lumpur, Malaysia
Publication Title
Electrophoresis
Volume
39
Issue
18
Publisher
Wiley-VCH Verlag
Additional Information
This concept has been submitted for patent filing in Malaysia (PI2014700972).