Morphological and Molecular Evidence for Multiple Taxa in Simulium parahiyangum (Diptera: Simuliidae), a Geographic Generalist Black Fly in Southeast Asia

Document Type

Article

Publication Date

1-1-2018

Abstract

Seven populations of Simulium parahiyangum Takaoka & Sigit (Diptera: Simuliidae), a geographically widespread nominal species of black fly in Southeast Asia, were morphologically and molecularly studied. Three morphoforms based on male and pupal morphological features, and two primary lineages based on the COI gene sequence analysis were recognized. Morphoform 1 (lineage 1) from Sarawak, Malaysia, is identified as S. parahiyangum sensu stricto and morphoform 2 (lineage 2) from Thailand and Vietnam, and morphoform 3 (lineage 1) from Peninsular Malaysia are each regarded as distinct species, although morphoform 3 is partially homosequential for the COI gene with morphoform 1. Morphoforms 2 and 3 are described as Simulium ngaoense sp. nov. and Simulium sazalyi sp. nov., respectively. Overall, S. parahiyangum is not a single geographic generalist but a composite of multiple species.

Keywords

black fly, COI gene, phylogenetics, Southeast Asia

Divisions

InstituteofBiologicalSciences

Funders

Research grant from University of Malaya (RP021A/16SUS),Thailand Research Fund,Office of the Higher Education Commission through the Research Grant for New Scholar (grant MRG5980-101),Diamond Research Grant from the Faculty of Medicine, Chiang Mai University, Thailand

Publication Title

Journal of Medical Entomology

Volume

55

Issue

6

Publisher

Oxford University Press

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