Immobilization of Bovine Serum Albumin on the Chitosan/PVA Film
Document Type
Article
Publication Date
1-1-2018
Abstract
Chitosan/polyvinyl alcohol (Chitosan/PVA) blended film was prepared by direct blend process and solution casting methods. In order to reduce the swelling ratio and enhance the chemical and mechanical stability, Chitosan/PVA film was crosslinked with glutaraldehyde in order to produce Chitosan-g-PVA. Bovine serum albumin (BSA) was used as a model protein to incorporate into the Chitosan-g-PVA. The chemical structure and morphological characteristics of films were studied by FT-IR and scanning electron microscopy (SEM). Mechanical and physical properties of blended films such as tensile properties in the dry and wet states, water uptake and water contact angle measurement were characterized. Blending PVA and chitosan improved strength and flexibility of the films. Crosslinking with glutaraldehyde further improves the tensile strength and decrease the hydrophilicity of films. BSA immobilized on the Chitosan-g-PVA film was calculated as BSA encapsulation efficiency.
Keywords
Bovine serum albumin, Chitosan, Glutaraldehyde, Polyvinyl alcohol
Divisions
fac_eng
Funders
University of Malaya Research (Grant RP034A-15AET) and Fundamental Research Grant Scheme (FRGS-FP026-2014B)
Publication Title
Sains Malaysiana
Volume
47
Issue
6
Publisher
Penerbit Universiti Kebangsaan Malaysia