Identifying protein biomarkers in predicting disease severity of dengue virus infection using immune-related protein microarray
Document Type
Article
Publication Date
1-1-2018
Abstract
Dengue virus is one of the most widespread flaviviruses that re-emerged throughout recent decades. The progression from mild dengue to severe dengue (SD) with the complications such as vascular leakage and hemorrhage increases the fatality rate of dengue. The pathophysiology of SD is not entirely clear. To investigate potential biomarkers that are suggestive of pathogenesis of SD, a small panel of serum samples selected from 1 healthy individual, 2 dengue patients without warning signs (DWS-), 2 dengue patients with warning signs (DWS+), and 5 patients with SD were subjected to a pilot analysis using Sengenics Immunome protein array. The overall fold changes of protein expressions and clustering heat map revealed that PFKFB4, TPM1, PDCL3, and PTPN20A were elevated among patients with SD. Differential expression analysis identified that 29 proteins were differentially elevated greater than 2-fold in SD groups than DWS- and DWS+. From the 29 candidate proteins, pathways enrichment analysis also identified insulin signaling and cytoskeleton pathways were involved in SD, suggesting that the insulin pathway may play a pivotal role in the pathogenesis of SD.
Keywords
dengue, hemorrhagic dengue fever, insulin pathway, plasma markers, protein array, severe dengue, TPM1
Divisions
fac_med
Funders
University Malaya Research Grant RP042A-15HTM and RP042B-15HTM
Publication Title
Medicine
Volume
97
Issue
5
Publisher
Lippincott, Williams & Wilkins