Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71
Document Type
Article
Publication Date
1-1-2018
Abstract
Enterovirus A71 (EV-A71) neutralization escape mutants were generated with monoclonal antibody MAB979 (Millipore). The VP2-T141I and VP1-D14N substitutions were identified. Using reverse genetics, infectious clones with these substitutions were constructed and tested by neutralization assay with immune sera from mice and humans. The N-terminus VP1-14 is more important than EF loop VP2-141 in acute human infection, mainly because it recognised IgM present in acute infection. The N-terminus VP1 could be a useful target for diagnostics and therapeutic antibodies in acute infection.
Keywords
capsid, enterovirus, enterovirus A71, geneticshand, foot, and mouth disease, immune responses, mutation, neutralization
Divisions
fac_med
Funders
Special Research Fund, University of Malaya BKS006-2017,Postgraduate Research Grant, University of Malaya PG157-2015B
Publication Title
Journal of Medical Virology
Volume
90
Issue
6
Publisher
Wiley