Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71

Authors

• Nik Mohd Nasir Nik Nadia
• Chee Wah Tan
• Kien Chai OngFollow
• I-Ching SamFollow
• Yoke Fun ChanFollow

Document Type

Article

Publication Date

1-1-2018

Abstract

Enterovirus A71 (EV-A71) neutralization escape mutants were generated with monoclonal antibody MAB979 (Millipore). The VP2-T141I and VP1-D14N substitutions were identified. Using reverse genetics, infectious clones with these substitutions were constructed and tested by neutralization assay with immune sera from mice and humans. The N-terminus VP1-14 is more important than EF loop VP2-141 in acute human infection, mainly because it recognised IgM present in acute infection. The N-terminus VP1 could be a useful target for diagnostics and therapeutic antibodies in acute infection.

Keywords

capsid, enterovirus, enterovirus A71, geneticshand, foot, and mouth disease, immune responses, mutation, neutralization

Publication Title

Journal of Medical Virology

Divisions

fac_med

Funders

Special Research Fund, University of Malaya BKS006-2017,Postgraduate Research Grant, University of Malaya PG157-2015B

Volume

90

Issue

6

Publisher

Wiley