Enrichment, in vitro, and quantification study of antidiabetic compounds from neglected weed Mimosa pudica using supercritical CO2 and CO2-Soxhlet
Document Type
Article
Publication Date
1-1-2018
Abstract
Supercritical fluid extraction (SFE) using carbon dioxide (CO2) and liquid CO2 using Soxhlet (CO2-Soxhlet) extraction were employed to extract three (3) antidiabetic compounds viz. stigmasterol, quercetin, and avicularin from Mimosa pudica. Various extraction parameters were studied. Extracts were analyzed pharmacologically, qualitatively and quantitatively to ascertain enrichment levels. All three antidiabetic compounds were effectively enriched under optimized conditions of temperature 60°C, pressure 40 MPa, co-solvent ratio 30%, and CO2 flow rate of 5 ml min−1. SFE was found to be the better method for enrichment of the antidiabetic compounds than the CO2-Soxhlet method. Extraction conditions were seen to affect the enrichment of desired compounds.
Keywords
Mimosa pudica, supercritical fluid extraction (SFE), CO2-Soxhlet, antidiabetic compounds, α-glucosidase assay
Divisions
nanocat
Funders
The exploratory research grant scheme, no. ERGS13-028-0061 of Ministry of Higher Education, Malaysia,International Scientific Partnership Program (ISPP) at King Saud University, Riyadh, Saudi Arabia: ISPP# 0026,Research Initiative Grant Scheme (RIGS16-303-0467) of International Islamic University Malaysia
Publication Title
Separation Science and Technology
Volume
53
Issue
2
Publisher
Taylor & Francis