Amperometric determination of L-cysteine using a glassy carbon electrode modified with palladium nanoparticles grown on reduced graphene oxide in a Nafion matrix
Document Type
Article
Publication Date
1-1-2018
Abstract
An amperometric sensor for L-Cys is described which consists of a glassy carbon electrode (GCE) that was modified with reduced graphene oxide placed in a Nafion film and decorated with palladium nanoparticles (PdNPs). The film was synthesized by a hydrothermal method. The PdNPs have an average diameter of about 10 nm and a spherical shape. The modified GCE gives a linear electro-oxidative response to L-Cys (typically at +0.6 V vs. SCE) within the 0.5 to 10 μM concentration range. Other figures of merit include a response time of less than 2 s, a 0.15 μM lower detection limit (at signal to noise ratio of 3), and an analytical sensitivity of 1.30 μA·μM−1·cm−2. The sensor displays selectivity over ascorbic acid, uric acid, dopamine, hydrogen peroxide, urea, and glucose. The modified GCE was applied to the determination of L-Cys in human urine samples and gave excellent recoveries.
Keywords
Cyclic voltammetry, Electrochemical sensor, Graphene materials, Hydrothermal synthesis, Metal nanoparticles, Nanocomposites, Urine sample
Divisions
Science
Funders
Postgraduate Research Fund (PG122-2014b) from the University of Malaya,Fundamental Research Grant Scheme (R/FRGS/A08.00/00648A/001/2016/000368) from Ministry of Higher Education
Publication Title
Microchimica Acta
Volume
185
Issue
4
Publisher
Springer Verlag (Germany)