A screen printed carbon electrode modified with carbon nanotubes and gold nanoparticles as a sensitive electrochemical sensor for determination of thiamphenicol residue in milk
Document Type
Article
Publication Date
1-1-2018
Abstract
Antibiotic residues in milk are of great concern for health regulatory agencies, milk consumers, and dairy farmers due to their destructive effects, ranging from allergic reactions, antibiotic resistance and the ability to interfere with the production of fermented products (i.e. cheese and yogurt). Therefore, a reliable, fast, and simple method needs to be developed to monitor antibiotic residues in milk samples before distribution to consumers. In this study, the first sensitive electrochemical sensor is presented for the determination of thiamphenicol (TAP), a broad-spectrum antibiotic in bovine milk. In the fabrication process, a screen printed electrode (SPE) was modified with gold nanoparticles (AuNPs) and carbon nanotubes (CNTs) using ethylenediamine (en) as a cross linker. Cyclic voltammetry studies showed an adsorptive control process for the electro-oxidation of TAP at-0.1 V on the modified electrode of SPE/CNT/en/AuNPs. Differential pulse voltammetry (DPV) was applied for the quantitative determination of TAP under optimized conditions (0.1 M citrate buffer, pH 6.0, accumulation potential-0.7 V, and accumulation time 150 s). A DPV study for TAP shows a wide linear calibration range of 0.1-30 μM with the detection limit of 0.003 μM. Furthermore, the developed sensor displays high sensitivity, reproducibility, repeatability, and good stability for the detection of TAP. The proposed sensor was successfully applied for the determination of spiked TAP in bovine milk with satisfactory results.
Keywords
Antibiotic resistance, Broad spectrum antibiotics, Differential pulse voltammetry, Ethylenediamine (en), Optimized conditions, Quantitative determinations, Screen printed electrodes, Screen-printed carbon electrodes
Divisions
CHEMISTRY
Funders
Universiti Putra Malaysia (Grant Putra 9142700),University of Malaya (UMRG RP020C-2016C)
Publication Title
RSC Advances
Volume
8
Issue
5
Publisher
Royal Society of Chemistry