Document Type
Article
Publication Date
12-20-2017
Abstract
INTRODUCTION: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. METHODS: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively. RESULTS: The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water. CONCLUSIONS: Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.
Keywords
Leptospira, Leptospirosis, Duplex endpoint, PCR
Divisions
InstituteofBiologicalSciences
Funders
University of Malaya, Kuala Lumpur, Malaysia: Research grant (RG053/11BIO & RP016B-14AFR) and Malaya High Impact Research Grant (reference UM.C/625/1HIR/MOHE/CHAN/11/2)
Publication Title
Revista da Sociedade Brasileira de Medicina Tropical
Volume
50
Issue
2
Publisher
Sociedade Brasileira de Medicina Tropical (SBMT)