Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling
Document Type
Article
Publication Date
1-1-2017
Abstract
With a diverse host range, Meloidogyne incognita (root-knot nematode) is listed as one of the most economically important obligate parasites of agriculture. This nematode species establishes permanent feeding sites in plant root systems soon after infestation. A compatible host-nematode interaction triggers a cascade of morphological and physiological process disruptions of the host, leading to pathogenesis. Such disruption is reflected by altered gene expression in affected cells, detectable using molecular approaches. We employed a high-throughput proteomics approach to elucidate the events involved in a compatible banana-M. incognita interaction. This study serves as the first crucial step in developing natural banana resistance for the purpose of biological-based nematode management programme. We successfully profiled 114 Grand naine root proteins involved in the interaction with M. incognita at the 30th- and 60th- day after inoculation (dai). The abundance of proteins involved in fundamental biological processes, cellular component organisation and stress responses were significantly altered in inoculated root samples. In addition, the abundance of proteins in pathways associated with defence and giant cell maintenance in plants such as phenylpropanoid biosynthesis, glycolysis and citrate cycle were also implicated by the infestation.
Keywords
Animals, Chromatography, Liquid, High-Throughput Screening Assays, Musa, Proteome, Tandem Mass Spectrometry, Tylenchoidea
Divisions
InstituteofBiologicalSciences
Funders
Ministry of Science, Technology and Innovation Malaysia, under Top-down Grant [number 53-02-03-1069, 2010],University of Malaya, Kuala Lumpur, Malaysia under Postgraduate research Grant [number PG194-2014B, 2014], and UMRG grant [number RP005E-13BIO, 2013],University of Malaya: Flagship programme [number FL002-2010, 2010]
Publication Title
PLoS ONE
Volume
12
Issue
6
Publisher
Public Library of Science