Document Type
Article (Restricted)
Publication Date
1-1-2011
Abstract
Bacterial secreted proteins are known to be involved in virulence and may mediate important host-pathogen interactions. In this study, when the stationary phase culture supernatant of Burkholderia pseudomallei was subjected to 2-DE, 113 protein spots were detected. Fifty-four of the secreted proteins, which included metabolic enzymes, transcription/translation regulators, potential virulence factors, chaperones, transport regulators, and hypothetical proteins, were identified using MS and database search. Twelve of these proteins were apparently reactive to antisera of mice that were immunised with B. pseudomallei secreted proteins. These proteins might be excellent candidates to be used as diagnostic markers or putative candidate vaccines against B. pseudomallei infections.
Keywords
Burkholderia pseudomallei, Immunoreactive, Proteomics, Secreted proteins
Divisions
fac_med
Publication Title
Electrophoresis
Volume
32
Issue
2
Additional Information
Correspondence: Professor Jamuna Vadivelu, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: jamuna@um.edu.my Fax: 01603-7967-6664