Species identification of intestinal microsporidia using immunofluorescence antibody assays
Document Type
Article
Publication Date
1-1-2011
Abstract
The species identification of Enterocytozoon bieneusi and Encephalitozoon intestinalis is only possible using transmission electron microscopy (TEM), molecular techniques and immunofluorescence antibody assays (IFA). In this study, 50 positive and 50 negative fecal specimens for microsporidial spores using the Weber modified trichrome (WMT) staining technique were examined using IFA-MAbs. Of the 100 specimens examined, the microsporidial spores identified by IFA-MAbs were Enterocytozoon bieneusi 42 (75) Encephalitozoon intestinalis 7(12.5) and mixed infections 7 (12.5). The sensitivity and specificity of IFA-MAbs in detecting microsporidial spores were 98 and 86, respectively. The agreement between the WMT staining technique and IFA-MAbs was statistically significant by Kappa statistics (K=0.840; p<0.001). E. bieneusi was the commonest Microsporidia species isolated from the studied population; the presence of microsporidial spores detected by IFA-MAbs should be confirmed by other methods.
Keywords
microsporidia, identification, IFA-MAbs
Publication Title
The Southeast Asian Journal of Tropical Medicine and Public Health
Volume
42
Issue
1
Publisher
SEAMEO Regional Tropical Medicine and Public Health Network
Additional Information
Al-Mekhlafi, M. Abdulsalam Fatmah, M. S. Anisah, N. 'Azlin, M. Al-Mekhlafi, Hesham M. Norhayati, M.