Real-time cell electrophysiology using a multi-channel dielectrophoretic-dot microelectrode array
Document Type
Article
Publication Date
1-1-2011
Abstract
Dielectrophoresis (DEP) has been used for many years for the analysis of the electrophysiological properties of cells. However, such analyses have in the past been time-consuming, such that it can take 30min or more to collect sufficient data to make valid interpretations from a single DEP spectrum. This has limited the application of the technology to a rapid tool for non-invasive, label-free research in areas from drug discovery to diagnostics. In this paper we present the development of a programmable, multi-channel DEP system for rapid biophysical assessment of populations of biological cells. A new assay format has been developed for continuous near-real-time monitoring, using simultaneous application of up to eight alternating current electrical signals to independently addressable dot microelectrodes in an array format, allowing a DEP spectrum to be measured in 20s, with a total cycle time between measurements of 90s. To demonstrate the system, human leukaemic K562 cells were monitored after exposure to staurosporine and valinomycin. The DEP response curves showed the timing and manner in which the membrane properties changed for the actions of these two drugs at the early phase of induction. This technology shows the great potential for increasing our understanding of the role of electrophysiology in drug action, by observing the changes in electrical characteristics as they occur. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Keywords
Characterisation Dielectrophoresis Microarrays Multi-frequency Real time staurosporine valinomycin article cell membrane cell population cell structure computer program controlled study electrophoresis electrophysiology human human cell information processing leukemia cell microarray analysis microelectrode Cell Line, Tumor Cell Physiological Phenomena Cytological Techniques Electrophysiological Phenomena Humans Microelectrodes Microfluidic Analytical Techniques
Divisions
fac_eng
Publication Title
Electrophoresis
Volume
32
Issue
18
Publisher
Wiley-VCH Verlag
Additional Information
Export Date: 11 December 2012 Source: Scopus CODEN: ELCTD PubMed ID: 21922496 Language of Original Document: English Correspondence Address: Labeed, F.H.; Centre for Biomedical Engineering, School of Engineering, University of Surrey, Guildford, Surrey GU2 7XH, United Kingdom; email: f.labeed@surrey.ac.uk Chemicals/CAS: staurosporine, 62996-74-1; valinomycin, 2001-95-8; Staurosporine, 62996-74-1; Valinomycin, 2001-95-8 References: Pethig, R., (2010) Biomicrofluidics, 4, p. 022811. , 1-35; Labeed, F.H., Coley, H.M., Thomas, H., Hughes, M.P., (2003) Biophys. J., 85, pp. 2028-2034; Jones, T.B., (2003) IEEE Eng. Med. Bio., 22, pp. 33-42; Pethig, R., Markx, G.H., (1997) Trends Biotech., 15, pp. 426-431; Ramos, A., Morgan, H., Green, N.G., Castellanos, A., (1998) Phys. D: Appl. Phys., 31, pp. 2228-2353; Castellanos, A., Ramos, A., Gonzalez, A., Green, N.G., Morgan, H., (2003) Phys. D: Appl. Phys., 36, pp. 2584-2597; Hughes, M.P., (2002) Electrophoresis, 23, pp. 2569-2582; Pethig, R., Lee, R.S., Talary, M.S., (2004) JALA, 9, pp. 324-330; Gascoyne, P.R.C., Vykoukal, J., (2002) Electrophoresis, 23, pp. 1973-1983; Goater, A.D., Burt, J.P.H., Pethig, R., (1997) Phys. D: Appl. Phys., 30, pp. L65; Cen, E.G., Dalton, C., Li, Y., Adamia, S., Pilarski, L.M., Kaler, K.V.I.S., (2004) J. Microbiol. Methods, 58, pp. 387-401; Medoro, G., Manaresi, N., Tartagni, M., Altomare, L., Leonardi, A., Guerrieri, R., (2002), 16 th European Conference on Solid State Transducers. Prague Doh, I., Cho, Y.H., (2005) Sensor Actuat. A - Phys., 121, pp. 59-65; Demierre, N., Braschler, T., Muller, R., Renaud, P., (2008) Sensor Actuat. B - Chem., 132, pp. 388-396; Medoro, G., Ma naresi, A., Leonardi, A., Altomare, L., Tartagni, M., Guerrieri, R., (2003) IEEE Sensor J., 3, pp. 317-325; Gascoyne, P.R.C., Vykoukal, J., (2004) Proc. IEEE, 92, pp. 22-42; Pohl, H.A., Crane, J.S., (1971) Biophys. J., 11, pp. 711-727; Hughes, M.P., (2003) Nanoelectromechanics in Engineering and Biology, , CRC Press, Boca Raton, FL, USA ; Jones, T.B., (1995) Electromechanics of Particles, , Cambridge University Press, Cambridge ; Auerswald, J., Knapp, H.F., (2003) Microelectron. Eng., 68, pp. 879-886; Kang, Y., Cetin, B., Wu, Z., Li, D., (2009) Electrochim. Acta, 54, pp. 1715-1720; Li, Y., Kaler, K.V.I.S., (2004) Anal. Chim. Acta., 507, pp. 151-161; Markx, G.H., Dyda, P.A., Pethig, R., (1996) J. Biotech., 51, pp. 175-180; Markx, G.H., Huang, Y., Zhou, X.-F., Pethig, R., (1994) Microbiol., 140, pp. 585-591; Markx, G.H., Pethig, R., (1995) Biotech. Bioeng., 45, pp. 337-343; Chan, K.L., Morgan, H., Morgan, E., Cameron, I.T., Thomas, M.R., (2000) BBA - Mol. Basis Dis., 1500, pp. 313-322; Gascoyne, P.R.C., Pethig, R., Satayavivad, J., Becker, F.F., Ruchirawat, M., (1997) BBA - Biomembranes, 1323, pp. 240-252; Wang, X., Becker, F.F., Gascoyne, P.R.C., (2002) BBA - Biomembranes, 1564, pp. 412-420; Broche, L.M., Bhadal, N., Lewis, M.P., Porter, S., Hughes, M.P., Labeed, F.H., (2007) Oral Oncol., 43, pp. 199-203; Chin, S., Hughes, M.P., Coley, H.M., Labeed, F.H., (2006) Int. J. Nanomed., 1, pp. 333-337; Fatoyinbo, H.O., Hoettges, K.F., Hughes, M.P., (2008) Electrophoresis, 1, pp. 3-10; Hoettges, K.F., Wiliams, R.M., Homewood, K.P., Stevenson, D., (2001) Chromotographia, 53, pp. S424-S426; Freshney, R., (1987) Culture of Animal Cells: A Manual of Basic Technique, , Alan R.Liss, Inc., New York ; Broche, L.M., Labeed, F.H., Hughes, M.P., (2005) Phys. Med. Biol., 50, pp. 1-8; Irimajiri, A., Hanai, T., Inouye, A., (1979) J. Theor. Biol., 78, pp. 251-269; Hoettges, K.F., Hubner, Y., Broche, L.M., Ogin, S.L., Kass, G.E.N., Hughes, M.P., (2008) Anal. Chem., 80, pp. 2063-2068; Lagarias, J.C., Reeds, J.A., Wright, M.H., Wright, P.E., (1998) SIAM J. Optim., 9, pp. 112-147; Nelder, J.A., Mead, R., (1965) Comput. J., 7, pp. 308-313; Vermes, I., Haanen, C., Steffens-Nakken, H., Reutellingsperger, C., (1995) J. Immunol. Methods, 184, pp. 39-51; Labeed, F.H., Coley, H.M., Hughes, M.P., (2006) BBA - Gen. Subjects, 1760, pp. 922-929; Inai, Y., Yabuki, M., Kanno, T., Akiyama, J., Yasuda, T., Utsumi, K., (1997) Cell Struct. Funct., 22, pp. 555-563; Burg, E.D., Remillard, C.V., Yuan, J.X.-J., (2006) J. Mem. Biol., 209, pp. 3-20; Furlong, I.J., Mediavilla, C.L., Ascaso, R., Rivas, A.L., Collins, M.K.L., (1998) Cell Death Differ., 5, pp. 214-221