Transcriptomic evaluation of plant growth inhibitory activity of goniothalamin from the Malaysian medicinal plant goniothalamus andersonii
Document Type
Article
Publication Date
1-1-2015
Abstract
Goniothalamin produced by the Malaysian medicinal plant, Goniothalamus andersonii J. Sinclair, strongly inhibits plant growth. However, its mode of action has not been characterized at the gene expression level. We conducted DNA microarray assay to analyze the changes in early gene responses of Arabidopsis thaliana seedlings. After a 6-h exposure to goniothalamin, we observed an upregulation of genes highly associated with heat response, and 22 heat shock protein (AtHSP) genes were upregulated more than 50 fold. Together with these genes, we observed upregulation of the genes related to oxidative stress and protein folding. Also, the genes related to cell wall modification and cell growth, expansin (AtEXPA) genes, were significantly downregulated. The results suggested that goniothalamin induces oxidative stresses and inhibits the expression of cell wall-associated proteins resulting in growth inhibition of Arabidopsis seedlings.
Keywords
DNA microarray, Expansins, Goniothalamin, Heat shock proteins, Plant growth inhibitory, Transcriptome, Unsaturated carbonyl groups
Divisions
InstituteofBiologicalSciences
Publication Title
Natural Product Communications
Volume
10
Issue
5
Publisher
SAGE Publications