Pseudo-nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA
Document Type
Article
Publication Date
1-1-2015
Abstract
An attempt was made to infer the phylogeny of Pseudo-nitzschia species by using the mitochondrial-encoded gene, cytochrome c oxidase subunit I (cox1), and comparing it with the nuclear-encoded large subunit ribosomal DNA (LSU rDNA). A pair of primers targeting Pseudo-nitzschia cox1 was designed in silico and used to infer the molecular phylogeny of Pseudo-nitzschia. The primer pair was tested using genomic DNAs isolated from six species of Pseudo-nitzschia from Malaysia. The phylogenetic inference of cox1 was then compared to the LSU rDNA phylogeny. Phylogenetic reconstructions of both data sets revealed monophyly of Pseudo-nitzschia species complexes. The range of genetic divergences among Pseudo-nitzschia species were higher in the cox1 data set (3.5–20.4 %) compared to the LSU rDNA data set (0.1–8.8 %). The present study suggests that high genetic divergence in cox1 of Pseudo-nitzschia species could be a useful genetic marker for DNA bar coding.
Keywords
cox1, Diatom, LSU rDNA, Primer, Pseudo-nitzschia
Divisions
Science
Funders
Malaysian Government through the Ministry of Education under KPM-JSPS COMSEA Matching Fund (GA002-2014),Higher Institution Centre of Excellence Research Grant (IOES-2014C),University of Malaya Research Program (RU006A/B-2014)
Publication Title
Fisheries Science
Volume
81
Issue
5
Publisher
Springer Verlag (Germany)