Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey
Document Type
Article
Publication Date
1-1-2015
Abstract
Honey was found to quench the intrinsic fluorescence of bovine serum albumin (BSA) in a concentration dependent manner, showing complete quenching in the presence of 5% (w/v) honey. Increasing the protein concentration up to 5.0 μM did not lead to the recovery of the protein fluorescence. Urea denaturation of BSA, which otherwise shows a two-step, three-state transition, using intrinsic fluorescence of the protein as the probe failed to produce any result in the presence of 5% (w/v) honey. Thus, intrinsic fluorescence cannot be used as a spectral probe for protein denaturation studies in the presence of honey.
Keywords
Bovine serum albumin, Fluorescence spectroscopy, Fluorimetric interference, Honey, Protein denaturation
Divisions
InstituteofBiologicalSciences
Publication Title
Journal of Applied Spectroscopy
Volume
82
Issue
5
Publisher
Kluwer (now part of Springer)