Inferred metagenomic comparison of mucosal and fecal microbiota from individuals undergoing routine screening colonoscopy reveals similar differences observed during active inflammation
Document Type
Article
Publication Date
1-1-2015
Abstract
The mucosal microbiota lives in close proximity with the intestinal epithelium and may interact more directly with the host immune system than the luminal/fecal bacteria. The availability of nutrients in the mucus layer of the epithelium is also very different from the gut lumen environment. Inferred metagenomic analysis for microbial function of the mucosal microbiota is possible by PICRUSt. We recently found that by using this approach, actively inflamed tissue of ulcerative colitis (UC) patients have mucosal communities enriched for genes involved in lipid and amino acid metabolism, and reduced for carbohydrate and nucleotide metabolism. Here, we find that the same bacterial taxa (e.g. Acinetobacter) and predicted microbial pathways enriched in actively inflamed colitis tissue are also enriched in the mucosa of subjects undergoing routine screening colonoscopies, when compared with paired samples of luminal/fecal bacteria. These results suggest that the mucosa of healthy individuals may be a reservoir of aerotolerant microbial communities expanded during colitis.
Keywords
Acinetobacter, Colitis, Inflammation, Microbiome, Mucosa, Proteobacteria
Divisions
fac_med
Funders
Kevin and Marsha Keating Family Foundation,UM/MoHE High Impact Research (H-20001–00-E000061) grant from University of Malaya,Grants from the National Institutes of Health (AI093811 and AI094166),Broad Medical Research Program,University of Malaya Student Grant (PV024/2011B),Grants-in-Aid of Research (G2012161472) from Sigma Xi, The Scientific Research Society,AGA-Eli and Edythe Broad Student Research Fellowship
Publication Title
Gut Microbes
Volume
6
Issue
1
Publisher
Taylor & Francis