Genome-based Proteomic Analysis of Lignosus rhinocerotis (Cooke) Ryvarden Sclerotium
Document Type
Article
Publication Date
1-1-2015
Abstract
Lignosus rhinocerotis (Cooke) Ryvarden (Polyporales, Basidiomycota), also known as the tiger milk mushroom, has received much interest in recent years owing to its wide-range ethnobotanical uses and the recent success in its domestication. The sclerotium is the part with medicinal value. Using two-dimensional gel electrophoresis coupled with mass spectrometry analysis, a total of 16 non-redundant, major proteins were identified with high confidence level in L. rhinocerotis sclerotium based on its genome as custom mapping database. Some of these proteins, such as the putative lectins, immunomodulatory proteins, superoxide dismutase, and aegerolysin may have pharmaceutical potential; while others are involved in nutrient mobilization and the protective antioxidant mechanism in the sclerotium. The findings from this study provide a molecular basis for future research on potential pharmacologically active proteins of L. rhinocerotis.
Keywords
Lignosus rhinocerotis, Proteomic analysis, LC-MS, MALDI-MS, Proteins
Divisions
fac_med
Funders
Fundamental Research Grant Scheme (FRGS) FP029-2014A from the Government of Malaysia,Postgraduate Research Fund (PPP) PV024/2012A from University of Malaya, Malaysia
Publication Title
International Journal of Medical Sciences
Volume
12
Issue
1
Publisher
Ivyspring International Publisher