Effects of freezing on sperm velocity in four different age groups of jermasia buck
Document Type
Article
Publication Date
1-1-2013
Abstract
The present study was undertaken to investigate the effects of freezing on sperm velocity in four different age groups (young: <1 year; mature: e <= 1.0-2.5 years; old: e <= 2.5-4.5 years and very old: e <= 4.5 years) of Jermasia buck. Tris Citric Acid Egg Yolk Extender (TCAYE) was added to semen samples and cryopreserved using a programmable freezer prior to plunging into liquid nitrogen (-196 degrees C). The fresh extended and frozen-thawed semen were subjected to computer automated semen analyzer (CASA) for sperm velocity evaluation. Fresh extended sperm in mature age group showed significantly highest values for average path velocity (VAP) 76.73 +/- 1.24 mu m/s, straight line velocity (VSL) 55.30 +/- 1.05 mu m/s, curvilinear velocity (VCL) 144.31 +/- 2.38 mu m/s, amplitude of lateral head displacement (ALH) 6.59 +/- 0.10 mu m and beat cross frequency (BCF) 39.59 +/- 0.36Hz as compared to young, old and very old age groups of buck (P<0.05). Significantly (P<0.05) higher values for VAP, VSL, VCL and ALH were observed in fresh as compared to frozen-thawed sperm in all age groups of buck. Thus, age of bucks should be considered as one of the factors that contribute to better sperm velocity which might affect fertilizability of the sperm. Optimizing the freezing technique is also essential in order to obtain satisfactory results after artificial insemination.
Keywords
Age, Buck Computer automated semen analyzer (casa), Goat, Semen, Freezing, Brown-norway rat, Motility parameters, Semen, Cryopreservation, Subpopulations, Spermatozoa, Frozen, Goat, Fertility, Analyzer
Divisions
InstituteofBiologicalSciences
Publication Title
Indian Journal of Animal Research
Volume
47
Issue
1
Publisher
Agricultural Research Communication Centre
Additional Information
Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA