Document Type
Conference Item
Publication Date
10-1-2012
Abstract
Oxytetracycline (OTC) is one of the most widely used antibiotics in aquaculture worldwide because of its broad spectrum of activity. The prevalence of OTCresistant environmental bacteria poses a serious risk in human and veterinary medicine. In this study, we isolated 140 and 142 OTC-resistant bacteria (~60Ilg/ml) from Port Dickson (PO) and Port Klang (PK), respectively. Among these OTC-resistant bacteria, 79 isolates (25 from PO and 54 from PK) were highly resistant to OTC (~2401lg!ml). These 79 isolates were then separated into different OTUs by restriction fragment length polymorphism (RFLP) method using three restriction enzymes (Rsal, Cfot, DdeI). The different OTUs were identified via 16S rONA partial sequencing. The dominant OTC-resistant bacteria in both sites were Alteromonas spp., Pseudoalteromonas spp., Shew anella spp. and Vibrio spp. Further testing on the susceptibility of these OTC-resistant bacteria against different classes of antibiotics will be carried out since OTC resistance is known to have cross-resistance to other unrelated antibiotics.
Keywords
Oxytetracycline (OTC), Malaysia, Antibiotic, Bacteria
Divisions
Science
Event Title
2nd Conference for Regional Cooperation in Ocean and Earth Science Research in the South China Sea
Event Location
University of Malaya, Kuala Lumpur, Malaysia
Event Dates
21-24 October 2012
Event Type
conference