Document Type
Article
Publication Date
1-1-2012
Abstract
Quorum sensing controls the virulence determinants in most proteobacteria. In this work, the hexane, chloroform and methanol extracts of an Ayurveda spice, namely clove (Syzygium aromaticum), shown anti-quorum sensing activity. Hexane and methanol extracts of clove inhibited the response of C. violaceum CV026 to exogenously supplied N-hexanoylhomoserine lactone, in turn preventing violacein production. Chloroform and methanol extracts of clove significantly reduced bioluminescence production by E. coli [pSB1075] grown in the presence of N-(3-oxododecanoyl)-l-homoserine lactone. We demonstrated that clove extract inhibited quorum sensing-regulated phenotypes in Pseudomonas aeruginosa PA01, including expression of lecA::lux (by hexane extract), swarming (maximum inhibition by methanol extract), pyocyanin (maximum inhibition by hexane extract). This study shows that the presence of natural compounds that exhibit anti-quorum sensing activity in the clove extracts may be useful as the lead of anti-infective drugs.
Keywords
Ayurveda, bioluminescence, biosensors, Chromobacterium violaceum, lecA::lux, N-acylhomoserine lactones, Pseudomonas aeruginosa, pyocyanin, Syzygium aromaticum, swarming
Divisions
fac_med,InstituteofBiologicalSciences
Publication Title
Sensors
Volume
12
Issue
4
Publisher
MDPI
Additional Information
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia; E-Mails: thibavengdes@yahoo.com (T.K.); yinwaifong@yahoo.com (W.F.Y.).