Document Type
Article
Publication Date
1-1-2012
Abstract
This study investigates the effects of 2-phenyl-1-benzopyran-4-one (flavone) on DENV-2 infectivity in Vero cells. Virus adsorption and attachment and intracellular virus replication were investigated using a foci forming unit assay (FFUA) and quantitative RT-PCR, respectively. Addition of flavone (100 μg/mL) significantly increased the number of DENV-2 foci by 35.66 ± 1.52 and 49.66 ± 2.51 when added during and after virus adsorption to the Vero cells, respectively. The average foci size after 4 days of infection increased by 33 ± 2.11 and 89 ± 2.13. The DENV-2 specific RNA copy number in the flavone-treated infected cells increased by 6.41- and 23.1-fold when compared to the mock-treated infected cells. Flavone (100 μg/mL) did not promote or inhibit Vero cell proliferation. The CC50 value of flavone against Vero cells was 446 µg/mL. These results suggest that flavone might enhance dengue virus replication by acting antagonistically towards flavonoids known to inhibit dengue virus replication.
Keywords
Antiviral, Dengue, Flavones, Flavonoid, Molecule
Publication Title
Molecules
Divisions
fac_med,medbio
Volume
17
Issue
3
Publisher
MDPI
Additional Information
Suggested Citation: Zandi K, Lani R, Wong P-F, Teoh B-T, Sam S-S, Johari J, et al. Flavone Enhances Dengue Virus Type-2 (NGC Strain) Infectivity and Replication in Vero Cells. Molecules. 2012;17(3):2437-45.