Document Type
Article
Publication Date
1-1-2012
Abstract
This study investigates the effects of 2-phenyl-1-benzopyran-4-one (flavone) on DENV-2 infectivity in Vero cells. Virus adsorption and attachment and intracellular virus replication were investigated using a foci forming unit assay (FFUA) and quantitative RT-PCR, respectively. Addition of flavone (100 μg/mL) significantly increased the number of DENV-2 foci by 35.66 ± 1.52 and 49.66 ± 2.51 when added during and after virus adsorption to the Vero cells, respectively. The average foci size after 4 days of infection increased by 33 ± 2.11 and 89 ± 2.13. The DENV-2 specific RNA copy number in the flavone-treated infected cells increased by 6.41- and 23.1-fold when compared to the mock-treated infected cells. Flavone (100 μg/mL) did not promote or inhibit Vero cell proliferation. The CC50 value of flavone against Vero cells was 446 µg/mL. These results suggest that flavone might enhance dengue virus replication by acting antagonistically towards flavonoids known to inhibit dengue virus replication.
Keywords
Antiviral, Dengue, Flavones, Flavonoid, Molecule
Divisions
fac_med,medbio
Publication Title
Molecules
Volume
17
Issue
3
Publisher
MDPI
Additional Information
Suggested Citation: Zandi K, Lani R, Wong P-F, Teoh B-T, Sam S-S, Johari J, et al. Flavone Enhances Dengue Virus Type-2 (NGC Strain) Infectivity and Replication in Vero Cells. Molecules. 2012;17(3):2437-45.