Document Type
Article
Publication Date
1-1-2012
Abstract
We report the production and degradation of quorum sensing N-acyl-homoserine lactones by bacteria isolated from Malaysian montane forest soil. Phylogenetic analysis indicated that these isolates clustered closely to the genera of Arthrobacter, Bacillus and Pseudomonas. Quorum quenching activity was detected in six isolates of these three genera by using a series of bioassays and rapid resolution liquid chromatography analysis. Biosensor screening and high resolution liquid chromatography-mass spectrometry analysis revealed the production of N-dodecanoyl-L-homoserine lactone (C12-HSL) by Pseudomonas frederiksbergensis (isolate BT9). In addition to degradation of a wide range of N-acyl-homoserine lactones, Arthrobacter and Pseudomonas spp. also degraded p-coumaroyl-homoserine lactone. To the best of our knowledge, this is the first documentation of Arthrobacter and Pseudomonas spp. capable of degrading p-coumaroyl-homoserine lactone and the production of C12-HSL by P. frederiksbergensis.
Keywords
Arthrobacter, Bacillus, liquid chromatography mass spectrometry (LC-MS), N-acylhomoserine lactone, N-dodecanoyl-L-homoserine lactone, p-coumaroylhomoserine lactone, Pseudomonas frederiksbergensis, Quorum quenching, Quorum sensing, Rapid resolution liquid chromatography (RRLC)
Divisions
fac_med,InstituteofBiologicalSciences
Publication Title
Sensors
Volume
12
Issue
4
Publisher
MDPI
Additional Information
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; E-Mails: cluster1986@hotmail.com (T.-M.C.); yinwaifong@yahoo.com (W.-F.Y.)