Development of a Nested PCR for Environmental Detection of the Pathogenic free-living Amoeba Balamuthia mandrillaris
Document Type
Article
Publication Date
1-1-2011
Abstract
A DNA extraction and nested PCR method for detecting the pathogenic amoeba Balamuthia mandrillaris from the environment was developed. Sixteen of 17 Californian soil samples were positive compared with 0/44 from the United Kingdom. This approach will enable a greater understanding of B. mandrillaris ecology, geographic distribution, and public health risk.
Keywords
Balamuthia, DNA, ecology, molecular, PCR, protozoa, ribosomal, sequence
Divisions
fac_med
Publication Title
Journal of Eukaryotic Microbiology
Volume
58
Issue
3
Additional Information
Ahmad, Arine F. Andrew, Peter W. Kilvington, Simon
COinS