Amycolatopsis granulosa sp. nov., Amycolatopsis ruanii sp. nov. and Amycolatopsis thermalba sp. nov., thermophilic actinomycetes isolated from arid soils

Document Type

Article

Publication Date

1-1-2012

Abstract

The taxonomic positions of three thermophilic actinomycetes isolated from arid soil samples were established by using a polyphasic approach. The organisms had chemical and morphological features that were consistent with their classification in the genus Amycolatopsis. 16S rRNA gene sequence data supported the classification of the isolates in the genus Amycolatopsis and showed that they formed distinct branches in the Amycolatopsis methanolica subclade. DNA- DNA relatedness studies between the isolates and their phylogenetic neighbours showed that they belonged to distinct genomic species. The three isolates were readily distinguished from one another and from the type strains of species classified in the A. methanolica subclade based on a combination of phenotypic properties and by genomic fingerprinting. Consequently, it is proposed that the three isolates be classified in the genus Amycolatopsis as representatives of Amycolatopsis granulosa sp. nov. (type strain GY307T5NCIMB 14709T5NRRL B-24844T), Amycolatopsis ruanii sp. nov. (type strain NMG112T5NCIMB 14711T5NRRL B-24848T) and Amycolatopsis thermalba sp. nov. (type strain SF45T5NCIMB 14705T5NRRL B-24845T). © 2012 IUMS.

Keywords

Actinomycetales, DNA, Bacterial, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, Soil Microbiology

Divisions

InstituteofBiologicalSciences

Publication Title

International Journal of Systematic and Evolutionary Microbiology

Volume

62

Issue

Pt 6

Publisher

Society for General Microbiology

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