Document Type
Article (Restricted)
Publication Date
1-1-2011
Abstract
The modified preparation technique developed in the present study has provided an excellent chromosome metaphase spread from the whole body of larvae of the red hybrid tilapia Oreochromis mossambicus (Peters, 1852) X Oreochromis niloticus (Linnaeus, 1758). Unlike other chromosome preparation methods, this modified technique showed a reduction in cell loss while suspending cells during the dropping process. This modified technique can therefore be widely applied not only to various tilapias but also to other teleostean fishes for preparing chromosome spreads. The best treatment parameters for preparing good chromosome spreads from red hybrid tilapia were optimized as 0.01 concentration of colchicine for 4-6 hours, hypotonic 40 minutes treatment, fixation with carnoy solution at 3:1 ratio and a concentration of 10 Giemsa for 20 minutes.
Keywords
Chromosome, Diploids, Hybrid Tilapia, Metaphase Spread, Triploids, Rainbow-Trout, Irideus Gibbons, Fish, Differentiation, Perciformes, Colchicine, Induction, Karyotype, Evolution, Triploidy
Divisions
OralPathologyOralMedicinePeriodontology
Publication Title
Caryologia
Volume
64
Issue
2
Publisher
Florence Edizioni Firenze
Additional Information
Pradeep, Padmaja Jayaprasad Srijaya, Thekkeparambil Chandrabose Zain, Rosnah Binti Mohd Papini, Alessio Chatterji, Anil Kumar