Document Type
Article
Publication Date
1-1-2011
Abstract
Restriction enzymes SpeI and XbaI were used in a pulsed-field gel electrophoresis (PFGE) study for molecular characterization of 146 clinical Burkholderia pseudomallei isolates. The PFGE parameters were optimized to enable comparable, reproducible, and robust results. The optimized parameters for both SpeI and XbaI restriction enzymes used in this study were 200 V and a pulse time of 5 to 65 s for a 28-h runtime. Using SpeI, 9 different clusters were identified, whereas 6 clusters were identified by XbaI digestion, which exhibited 85 similarity to SpeI. SpeI (discrimination index D=0.854) showed higher discriminatory power than XbaI did (D=0.464).
Keywords
Molecular Medicine, Restriction enzymes SpeI and XbaI
Divisions
fac_med
Publication Title
Japanese Journal of Infectious Diseases
Volume
64
Issue
3
Publisher
Institute of Infectious Diseases
Additional Information
Corresponding author: Mailing address: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Tel: ＋603- 79676607, Fax: ＋603-79676600, E-mail: khchua＠um.edu.my