Document Type
Article
Publication Date
1-1-2011
Abstract
Glucoamylase (EC 3.2.1.3) from Aspergillus niger was used to hydrolyze commercial sago starch into reducing sugars. The energetics of the hydrolysis was studied at optimal substrate concentration, enzyme amount and pH for the process. The ΔH and ΔS estimated via van't Hof analysis were 7.9 J.mole -1 and 6.2 J.mole -1.K -1, respectively. The results indicated that the hydrolysis process is an endothermic reaction and spontaneous within the temperature range tested. It is calculated that 26.3 J.mole -1 of activation energy, E a is required for the enzymatic conversion of sago starch into reducing sugars. The spontaneous reaction with relatively low activation energy supported the viability of glucoamylase-catalyzed hydrolysis of sago starch.
Keywords
Energetics, Glucoamylase, Reducing sugars, Sago starch, Aspergillus niger.
Divisions
fac_eng
Publication Title
Asia Pacific Journal of Molecular Biology and Biotechnology
Volume
19
Issue
4
Publisher
Malaysian Society for Molecular Biology and Biotechnology
Additional Information
Export Date: 13 February 2014 Source: Scopus Language of Original Document: English Correspondence Address: Annuar, M. S. B. M.; Institute of Biological Sciences, Faculty of Science, Kuala Lumpur 50603, Malaysia; email: sufanannuar@um.edu.my References: Adinarayaan, K., Suren, S., Response surface optimization of enzymatic hydrolysis of maize starch for higher glucose production (2005) Biochemical Engineering Journal, 27, pp. 179-190; Carlos, R.S., Pappy, J.R., Anil, K.P., Woiciechowski, L., Luciana, P.S.V., Ashok, P., (2006), pp. 221-237. , Glucoamylase: In Ashok, P., Colin, W., Carlos, R.S. and Christian, L. (ed), Enzyme technology. India: Springer, Asiatech PublCornish-Bowden, A., (1972) Fundamentals of Enzyme Kinetic, , United Kingdom: Butterworth & Co. (Publ.) Ltd; Miller, G.L., Use of DNS reagent for determination of reducing sugars (1959) Analytical Chemistry, 31, pp. 426-428; Shuler, M.L., Kargi, F., (1992) Bioprocess Engineering: Basic Concepts, , USA: Prentice-Hall International; Silberberg, M.S., (2006) Chemistry: The Molecular Nature of Matter and Change, , 5th edn. New York: McGraw-Hill International, Inc; Singhal, R.S., Kennedy, J.F., Gopalakrishnan, S.M., Kacz-Marek, A., Knill, C.J., Akmar, P.F., Industrial production, processing, and utilization of sago palm-derived products (2008) Carbohydrate Polymers, 72, pp. 1-20; Suraini, A.A., Review: Sago starch and its Utilization (2002) Journal of Bioscience and Bioengineering, 94, pp. 526-529; Stanton, R., Have your trees and eat them (1993) Food Science and Technology Today, 7, pp. 89-94; Swinkels, J.J.M., Sources of starch, its chemistry and physics (1985) Starch Conversion Technology, pp. 15-45. , eds. G. M. A. van Beynum and J. A. Roels, Marcel Dekker, New York; Wang, W.J., Powell, A.D., Oates, C.G., Sago starch as a biomass source: Raw sago starch hydrolysis by commercial enzymes (1996) Bioresource Technology, 55, pp. 55-61; Wee, L.L., Annuar, M.S.M., Ibrahim, S., Chisti, Y., Enzyme-mediated production of sugars from sago starch: Statistical process optimization (2011) Chemical Engineering Communications, 198, pp. 1339-1353