Document Type
Article (Restricted)
Publication Date
1-1-2011
Abstract
Amongst HIV patients with successful virological responses to antiretroviral therapy (ART), poor CD4(+) T-cell recovery is associated with low nadir CD4(+) T-cell counts and persistent immune activation. These factors might be influenced by dendritic cell (DC) function. Interferon-alpha-producing plasmacytoid DC and IL-12-producing myeloid DC were quantified by flow cytometry after stimulation with agonists to TLR7/8 (CL075) or TLR9 (CpG-ODN). These were compared between patients who achieved CD4(+) T-cell counts above or below 200 cells/mu L after 6 months on ART (High vs. Low groups). High Group patients had more DC producing interferon-alpha or IL-12 at Weeks 6 and 12 on ART than Low Group patients. The frequencies of cytokine-producing DC at Week 12 were directly correlated with CD4(+) T-cell counts at baseline and at Week 12. Patients with good recovery of CD4(+) T-cells had robust TLR-mediated interferon-alpha responses by plasmacytoid DC and IL-12 responses by myeloid DC during early ART (1-3 months). (C) 2011 Elsevier Inc. All rights reserved.
Keywords
Antiretroviral therapy, HIV, Interferon-α, IL-12, Dendritic cells, Toll-like receptors
Divisions
fac_med
Publication Title
Clinical Immunology
Volume
139
Issue
2
Additional Information
Tan, Dino Bee Aik Yong, Yean Kong Lim, Andrew Tan, Hong Yien Kamarulzaman, Adeeba French, Martyn Price, Patricia