Document Type

Article

Publication Date

1-1-2011

Abstract

Tissue culture studies of Laivsonia inermis, syn. Lawsonia alba (henna) were carried out to induce multiple shoots, plant regeneration and coloured callus on MS media supplemented with various hormones. Various explants, such as sections of leaves, stems and roots, from 4-month-old aseptic seedlings were used. Different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), napthalene acetic acid (NAA), benzyl aminopurine (BAP), kinetin, and indole acetic acid (IAA) hormones were used. The results showed that efficient regeneration system for henna plant in vitro could be achieved. Different coloured callus were obtained from henna tissue culture on MS supplemented with different combinations and concentrations of hormones 0.5 mg/L 2,4-D and 0.5 mg/L IAA, 2.0 mg/L NAA and 2.0 mg/L BAP, 0.5 mg/L NAA and 0.5 ing/L BAP, 1.5 mg/L NAA and 1.5 mg/L BAP, and also 0.5 mg/L IAA and 0.5 mg/L BAP, and thus production of important secondary metabolites in vitro is possible for this important species. Formation of embryogenic callus was observed during the plant morphogenesis in MS medium supplemented with combination of 0.5 mg/L IAA and 0.5 mg/L 2,4-D hormones which will enhance more production of in vitro plantlets and ultimately promote new propagules of this species.

Keywords

Tissue culture Murashige and Skoog (MS) multiple shoot aseptic seedlings 2, 4-dichlorophenoxyacetic acid (2, 4-D) napthalene acetic acid (NAA) benzyl aminopurine (BAP) indole acetic acid (IAA) in vitro embryogenic callus cultures

Divisions

Science

Publication Title

Journal of Food Agriculture & Environment

Volume

9

Issue

2

Additional Information

Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA

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