Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization

Document Type

Article

Publication Date

10-1-2007

Abstract

Fluorescent in situ hybridization (FISH) was carried out using two different oligonucleotide probes specific for Pseudomonas spp. and Acinetobacter spp. These probes were tested against different organisms and were found to be highly specific. Sensitivity testing showed that the probes were able to detect as low as 10 3 CFU/mL. In addition, FISH was carried out directly on positive blood culture samples and the detection of microorganisms took less than 2 h. We believe that FISH is a rapid method that can be used as a routine laboratory diagnostic technique for the detection of Acinetobacter spp. and Pseudomonas spp. in clinical samples.

Keywords

Acinetobacter/genetics, Acinetobacter/isolation & purification

Publication Title

Indian Journal of Medical Microbiology

ISSN

0255-0857

Divisions

medbio

Volume

25

Issue

4

Publisher

Medknow Publications

Additional Information

Department of Medical Microbiology, Faculty of Medicine, University Malaya, 50603, Kuala Lumpur, Malaysia.

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