Document Type
Article
Publication Date
1-1-2007
Abstract
hypocotyls and cotyledons of Capsicum annuum var Kulai from 8-14 days old seedlings were cultured on MS medium supplemented with various concentrations of BAP and IAA. Effect of explant types, developmental stages of seedling and duration of subculture in different media were studied. Young tissue from hypocotyls formed more buds than the older tissue. Hypocotyls showed better response than cotyledons. Subculture was carried out every two weeks to prevent browning of explants and promote adventitious bud formation and development. Optimal bud forming efficiency in hypocotyl explants was obtained when explants were cultured on MS media containing 3 mg/l BAP and 0.3 mg/l IAA for 2 weeks followed by subculture on medium supplemented with 2mg/l BAP and 0.15mg/l IAA. The percentage of bud formation was 75.48 ± 4.71 .
Keywords
Adventitious bud formation, Capsicum annuum
Publication Title
Asia Pacific Journal of Molecular Biology and Biotechnology
ISSN
0128-7451
Recommended Citation
Mok, S.H. and Khalid, Norzulaani, "Trouble Shooting for Recalcitrant Bud Formation in Capsicum annuum var. Kulai" (2007). Research Publications (2006 to 2010). 754.
https://knova.um.edu.my/research_publications_2006_2010/754
Divisions
InstituteofBiologicalSciences
Volume
15
Issue
1
Publisher
Malaysian Society for Molecular Biology and Biotechnology