Document Type
Conference Item
Publication Date
1-1-2007
Abstract
Salmonella enterica continues to be an important foodborne pathogen worldwide. Serotyping is the fundamental approach in the epidemiology of Salmonella. It consists of the immunologic classification of two surface structures, O-polysaccharide (0 antigen) and flagellin protein (H antigen). Salmonella serotypes are the basis for national surveillance networks and are critical to the identification of outbreaks.The current conventional serotyping procedure is time-consuming, requiring 3 to 5 days or more to fully detennine the serotype, thus delaying infonnation and costing laboratories in overhead. Furthennore, production and quality control of the hundreds of antisera required for serotyping are difficult and time consuming. To avoid the difficulties of antisera production and to take advantage of the automability of DNA technology, the National Centre for Infectious Diseases, CDC, Atlanta has developed a system for the determination of serotypes based on DNA markers within genes responsible for 0 and H antigen expression. This system combines PCR with a bead-based suspension array detection system. The objective of this paper is to describe the application of the Luminex® Multianalyte profling (xMAP) technology to detect the H antigens of Salmonella.
Keywords
Salmonella enterica, Antigen expression, DNA, Genes, Hybridization
Divisions
InstituteofBiologicalSciences
Event Title
29th Symposium of the Malaysian Society for Microbiology
Event Location
Kuala Terengganu, Terengganu, Malaysia
Event Dates
24-26 November 2007
Event Type
conference
Additional Information
Conference paper