Document Type
Conference Item
Publication Date
1-1-2007
Abstract
Surveys were conducted between the years of 2005-2006 at several locations in the northern, central and southern parts of West Malaysia to study the polymorphism of R. solanacearum strains. These sites included vegetable and farms with known hosts of the pathogen, such as banana, tomato, eggplant, chili and tobacco. Sample collection was conducted by randomly collecting the suspected plants and weeds, including soil and water samples, from selected areas. The bacterium was detected in all samples by Nested-PCR. The bacteria were isolated from positive samples by using semi-selective media and were further confirmed by biochemical and BIOLOG test. FAME (Fatty Acid Methyl Esters) profiling? was performed for the detection of polymorphism among bacterial isolates. The results showed that the fatty acid composition varies for all R. solanacearum isolates. Grouping of R. solanacearum isolates by fatty acid composition suggested the existence of eight groups namely group A, B, C, D, E, F, G andH.
Keywords
Ralstonia solanacearum, Farms, Vegetable, Bacteria, Biochemical
Divisions
InstituteofBiologicalSciences
Event Title
29th Symposium of the Malaysian Society for Microbiology
Event Location
Kuala Terengganu, Terengganu, Malaysia
Event Dates
24-26 November 2007
Event Type
conference
Additional Information
Conference paper