Document Type
Article
Publication Date
1-1-2007
Abstract
The expression of high-abundance serum proteins in newly diagnosed patients with endometrial adenocarcinoma (EACa), squarnous cell cervical carcinoma (SCCa) and cervical adenocarcinorna (ACCa), relative to control female subjects, was analyzed by subjecting serum samples to 2-DE followed by image analysis of the silver-stained protein profiles. The three cohorts of cancer patients demonstrated different altered expression of serum high-abundance proteins compared to negative control women. Tl-ie expression of alpha(1)-antitrypsin, alpha(1)-B glycoprotein, cleaved high-molecular-weight kininogen (light chain) and antithrombin III were consistently altered in all the patients. However, clusterin was upregulated only in the patients with EACa, while those with SCCa and ACCa were typically characterized by the upregulated expression of zinc alpha-2-glycoprotein. The aberrant expression of selective serum proteins in the various cohorts of cancer patients was validated by competitive ELISA as well as by lectin detection. Analysis by using the charnpedak galactose binding lectin further highlighted an unidentified protein that may be differently glycosylated in the sera of the EACa patients that were studied.
Keywords
Cervical canceer, Endometrial cancer, Lectin, O-Glycans, Proteomics
Publication Title
Electrophoresis
ISSN
0173-0835
Recommended Citation
Abdul-Rahman, P.S.; Lim, B.K.; and Hashim, Onn Haji, "Expression of high-abundance proteins in sera of patients with endometrial and cervical cancers: analysis using 2-DE with silver staining and lectin detection methods" (2007). Research Publications (2006 to 2010). 670.
https://knova.um.edu.my/research_publications_2006_2010/670
Divisions
fac_med
Volume
28
Issue
12
Additional Information
Abdul-Rahman, Puteri S. Lim, Boon-Kiong Hashim, Onn H.